Enhanced Extraction of Bioactive Compounds from Bee Pollen ...
Bee pollen underwent wet grinding for 1 to 5 hr with a Rotate Mill (Ilsin Nanotechnology Co., Ltd., Japan). The particle size of the wetground bee pollen was determined with the photon correlation spectroscopy technique on a particlesize analyzer (Mastersizer 2000, Malvern, England), as described by Nair et al. [14].